Dr. John Bozzola
IMAGE Center 1013
Office Phone: 618-453-3730
email: bozzola at siu.edu
Assisted
by:
Dr. Stephen Schmitt
Ms. Hilary Gates
COURSE DESRIPTION
502-4 (2,2) Research Scanning Electron Microscopy. (a) Theory of design
for scanning electron microscope, lenses, vacuum systems, alignment,
specimen preparation for biologists and materials scientists, darkroom.
(b) Laboratory practical experience in use of scanning electron
microscope and specimen preparation. Laboratory fee $100.
TEXTBOOK (Recommended)
Bozzola, J.J. and Russell, L. 1999. Electron Microscopy: Principles
& Techniques. Jones & Bartlett Publishers (Sudbury, MA)
670
pages. ISBN # 0-7637-0192-0.
COURSE OBJECTIVES
SCI502 or PLB 502, Research Scanning Electron Microscopy, is a graduate
level course in the use of the scanning electron microscope as a
research tool. It is an introductory course designed to provide
sufficient background information to thoughtfully design research
protocols, prepare specimens, use instrumentation for the optimal
recording of images, and prepare the images for presentation at a
professional scientific meeting. The course is divided into 502A
(lecture) and 502B (lab). Both may be taken concomitantly or separately
if student has appropriate academic or practical experience. Upon
satisfactory completion of the introductory course, new users of the
facility will receive more, in-depth instruction on instrument use and
specimen preparation suited to your specific research project.
LABORATORY FEE
The laboratory fee
($150.00) is due by the fourth week of the course. If this poses
problems, divided
payments may be made throughout the course until the total amount is
paid before the end
of the course. This fee covers expenses related to the operation and
maintenance of the microscope
only.
COURSE INFORMATION
The purpose of the lab course is to train individuals to
use the
electron microscope in a research project at SIU. The student must have
a research project upon signing up for the course.
The student is expected to produce a high quality poster
and to
give a 15 min presentation of the research for the final lab
exam.
The poster becomes the property of the IMAGE for
several semesters
(to display and demo to other students and faculty). 50% of the
student's grade will be derived from the poster and the
presentation.
Being a graduate level course, the student is expected not
only
to become proficient in the basic use of the SEM and ancillary
equipment but also to understand the operational principles of
the microscope and all ancillary equipment. This will
necessitate
doing outside readings, answering questions and taking quizzes
related to principles. In other words, this is not simply a
“how
to” course, but requires understanding.
The overall product of any electron microscope is an
electron
micrograph made from a digital file. We will train you how to
do
this prior to the poster presentation.
Professional electron microscopists are by necessity neat,
fastidious and highly-organized individuals. We expect all students to
keep their work areas likewise. The appearance of the
poster (as
well as all micrographs and lab reports) must be well-organized, neat
and professional in appearance.
Any reports will be submitted as typewritten documents, and
will
be due when indicated by the instructor. Quizzes may consist
of
oral and possibly written questions related to the present or
past
labs.
Images for the laboratory reports will be produced
digitally or
on a thermal printer via an SEM image capture system. Images
for
the poster will be generated digitally. Students will
receive two
specimen stubs and a stub box.
50% of grade = laboratory reports. You will be permitted to
resubmit one lab a second time to improve your grade.
The recommended textbook for the course is Bozzola and
Russell.
Other acceptable books covering the topics discussed in the
course
are also listed below. These may be obtained from Morris
Library
or used in the library at the IMAGE Center.
SYLLABUS
For a pdf file of a past syllabus, click HERE
For the entire Laboratory Packet, click HERE.
SIUC / College of Science / Research Scanning Electron Microscopy
URL: http://www.science.siu.edu/PLB502/index.html
Last updated: 30-Aug-07 / dln
